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New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae

Gauss, R. and Trautwein, M. and Sommer, T. and Spang, A.. (2005) New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae. Yeast, 22 (1). pp. 1-12.

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Official URL: http://edoc.unibas.ch/dok/A5259747

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Abstract

Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)
UniBasel Contributors:Spang, Anne
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley
ISSN:0749-503X
e-ISSN:1097-0061
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Nov 2017 15:47
Deposited On:22 Mar 2012 13:31

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