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Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress

Devos, Simon and Van Putte, Wouter and Vitse, Jolien and Van Driessche, Gonzalez and Stremersch, Stephan and Van Den Broek, Wim and Raemdonck, Koen and Braeckmans, Kevin and Stahlberg, Henning and Kudryashev, Misha and Savvides, Savvas N. and Devreese, Bart. (2017) Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress. Environmental Microbiology, 19 (10). pp. 3930-3937.

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Official URL: http://edoc.unibas.ch/56399/

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Abstract

Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1462-2912
e-ISSN:1462-2920
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:20 Oct 2017 06:44
Deposited On:20 Oct 2017 06:44

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