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Cryo-EM structure of the extended type VI secretion system sheath-tube complex

Wang, Jing and Brackmann, Maximilian and Castaño-Díez, Daniel and Kudryashev, Mikhail and Goldie, Kenneth N. and Maier, Timm and Stahlberg, Henning and Basler, Marek. (2017) Cryo-EM structure of the extended type VI secretion system sheath-tube complex. Nature Microbiology, 2 (11). pp. 1507-1512.

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Official URL: http://edoc.unibas.ch/56324/

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Abstract

The bacterial type VI secretion system (T6SS) uses contraction of a long sheath to quickly thrust a tube with associated effectors across membranes of eukaryotic and bacterial cells. Only limited structural information is available about the inherently unstable precontraction state of the T6SS. Here, we obtain a 3.7 Å resolution structure of a non-contractile sheath–tube complex using cryo-electron microscopy and show that it resembles the extended T6SS inside Vibrio cholerae cells. We build a pseudo-atomic model of the complete sheath–tube assembly, which provides a mechanistic understanding of coupling sheath contraction with pushing and rotating the inner tube for efficient target membrane penetration. Our data further show that sheath contraction exposes a buried recognition domain to specifically trigger the disassembly and recycling of the T6SS sheath by the cognate ATP-dependent unfoldase ClpV.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Infection Biology (Basler)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Maier)
05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
05 Faculty of Science
UniBasel Contributors:Stahlberg, Henning and Maier, Timm and Basler, Marek and Goldie, Kenneth N.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
e-ISSN:2058-5276
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:01 Oct 2020 14:51
Deposited On:13 Oct 2017 08:00

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