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Solution structure of discoidal high-density lipoprotein particles with a shortened apolipoprotein A-I

Bibow, Stefan and Polyhach, Yevhen and Eichmann, Cédric and Chi, Celestine N. and Kowal, Julia and Albiez, Stefan and McLeod, Robert A. and Stahlberg, Henning and Jeschke, Gunnar and Güntert, Peter and Riek, Roland. (2017) Solution structure of discoidal high-density lipoprotein particles with a shortened apolipoprotein A-I. Nature Structural and Molecular Biology, 24 (2). pp. 187-193.

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Official URL: http://edoc.unibas.ch/56296/

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Abstract

High-density lipoprotein (HDL) particles are cholesterol and lipid transport containers. Mature HDL particles destined for the liver develop through the formation of intermediate discoidal HDL particles, which are the primary acceptors for cholesterol. Here we present the three-dimensional structure of reconstituted discoidal HDL (rdHDL) particles, using a shortened construct of human apolipoprotein A-I, determined from a combination of nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) and transmission electron microscopy (TEM) data. The rdHDL particles feature a protein double belt surrounding a lipid bilayer patch in an antiparallel fashion. The integrity of this structure is maintained by up to 28 salt bridges and a zipper-like pattern of cation-π interactions between helices 4 and 6. To accommodate a hydrophobic interior, a gross 'right-to-right' rotation of the helices after lipidation is necessary. The structure reflects the complexity required for a shuttling container to hold a fluid lipid or cholesterol interior at a protein:lipid ratio of 1:50.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:1545-9993
e-ISSN:1545-9985
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:25 Oct 2017 14:51
Deposited On:12 Oct 2017 09:20

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