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Focus: The interface between data collection and data processing in cryo-EM

Biyani, Nikhil and Righetto, Ricardo D. and McLeod, Robert and Caujolle-Bert, Daniel and Castano-Diez, Daniel and Goldie, Kenneth N. and Stahlberg, Henning. (2017) Focus: The interface between data collection and data processing in cryo-EM. Journal of Structural Biology, 198 (2). pp. 124-133.

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Official URL: http://edoc.unibas.ch/56292/

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Abstract

We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1047-8477
e-ISSN:1095-8657
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Oct 2017 09:23
Deposited On:12 Oct 2017 09:23

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