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The role of trimerization in the osmoregulated betaine transporter BetP

Perez, Camilo and Khafizov, Kamil and Forrest, Lucy R. and Krämer, Reinhard and Ziegler, Christine. (2011) The role of trimerization in the osmoregulated betaine transporter BetP. EMBO Reports, 12 (8). pp. 804-810.

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Official URL: http://edoc.unibas.ch/56048/

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Abstract

The osmoregulated betaine transporter BetP is a stable trimer. Structural studies have shown that individual protomers can adopt distinct transport conformations, implying a functional role for the trimeric state in transport, although the role of trimerization in regulation is not yet understood. We designed putative monomeric mutants by molecular-dynamics simulations and in silico alanine-scanning mutagenesis. Several mutants including BetP-W101A/T351A were monomeric in detergent as well as in the membrane, as shown by blue native gel electrophoresis, crosslinking and electron microscopy. This monomeric form retains the ability to accumulate betaine, but is no longer regulated by hyperosmotic shock.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics
UniBasel Contributors:Perez, Camilo
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:1469-221X
e-ISSN:1469-3178
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:30 Nov 2017 10:02
Deposited On:30 Nov 2017 10:02

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