edoc

The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals

Cringoli, Giuseppe and Maurelli, Maria P. and Levecke, Bruno and Bosco, Antonio and Vercruysse, Jozef and Utzinger, Jürg and Rinaldi, Laura. (2017) The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals. Nature Protocols, 12 (9). pp. 1723-1732.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/55884/

Downloads: Statistics Overview

Abstract

This protocol is an extension to: Nat. Protoc. 5, 503–515 (2010). DOI: 10.1038/nprot.2009.235;
The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Health Impact Assessment (Utzinger)
UniBasel Contributors:Utzinger, Jürg
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
ISSN:1754-2189
e-ISSN:1750-2799
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Oct 2017 10:29
Deposited On:19 Oct 2017 10:29

Repository Staff Only: item control page