Peripheral T cell cytokine responses for diagnosis of active tuberculosis

Nemeth, Johannes and Winkler, Heide-Maria and Zwick, Ralph H. and Müller, Catharina and Rumetshofer, Rudolf and Boeck, Lucas and Burghuber, Otto C. and Winkler, Stefan. (2012) Peripheral T cell cytokine responses for diagnosis of active tuberculosis. PLoS ONE, 7 (4). e35290.

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Official URL: http://edoc.unibas.ch/55864/

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A test for diagnosis of active Tuberculosis (TB) from peripheral blood could tremendously improve clinical management of patients.; Of 178 prospectively enrolled patients with possible TB, 60 patients were diagnosed with pulmonary and 27 patients with extrapulmonary TB. The frequencies of Mycobacterium tuberculosis (MTB) specific CD4(+) T cells and CD8(+) T cells producing cytokines were assessed using overnight stimulation with purified protein derivate (PPD) or early secretory antigenic target (ESAT)-6, respectively.; Among patients with active TB, an increased type 1 cytokine profile consisting of mainly CD4(+) T cell derived interferon (IFN)-γ was detectable. Despite contributing to the cytokine profile as a whole, the independent diagnostic performance of one cytokine producing T cells as well as polyfunctional T cells was poor. IFN-γ/Interleukin(IL)-2 cytokine ratios discriminated best between active TB and other diseases.; T cells producing one cytokine and polyfunctional T cells have a limited role in diagnosis of active TB. The significant shift from a "memory type" to an "effector type" cytokine profile may be useful for further development of a rapid immune-diagnostic tool for active TB.
Faculties and Departments:03 Faculty of Medicine > Bereich Medizinische Fächer (Klinik) > Pneumologie
03 Faculty of Medicine > Departement Klinische Forschung > Bereich Medizinische Fächer (Klinik) > Pneumologie
UniBasel Contributors:Boeck, Lucas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Public Library of Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Aug 2018 06:33
Deposited On:05 Oct 2017 07:09

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