Exploring the Role of PGC-1α in Defining Nuclear Organisation in Skeletal Muscle Fibres

Ross, Jacob Alexander and Pearson, Adam and Levy, Yotam and Cardel, Bettina and Handschin, Christoph and Ochala, Julien. (2017) Exploring the Role of PGC-1α in Defining Nuclear Organisation in Skeletal Muscle Fibres. Journal of Cellular Physiology, 232 (6). pp. 1270-1274.

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Official URL: http://edoc.unibas.ch/54531/

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Muscle fibres are multinucleated cells, with each nucleus controlling the protein synthesis in a finite volume of cytoplasm termed the myonuclear domain (MND). What determines MND size remains unclear. In the present study, we aimed to test the hypothesis that the level of expression of the transcriptional coactivator PGC-1α and subsequent activation of the mitochondrial biogenesis are major contributors. Hence, we used two transgenic mouse models with varying expression of PGC-1α in skeletal muscles. We isolated myofibres from the fast twitch extensor digitorum longus (EDL) and slow twitch diaphragm muscles. We then membrane-permeabilised them and analysed the 3D spatial arrangements of myonuclei. In EDL muscles, when PGC-1α is over-expressed, MND volume decreases; whereas, when PGC-1α is lacking, no change occurs. In the diaphragm, no clear difference was noted. This indicates that PGC-1α and the related mitochondrial biogenesis programme are determinants of MND size. PGC-1α may facilitate the addition of new myonuclei in order to reach MND volumes that can support an increased mitochondrial density.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Associated Research Groups > Pharmakologie (Handschin)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Growth & Development (Handschin)
UniBasel Contributors:Handschin, Christoph
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article -- The final publication is available at Springer via DOI link.
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Last Modified:03 Oct 2017 09:29
Deposited On:03 Mar 2017 09:00

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