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Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014

Van der Auwera, Gert and Bart, Aldert and Chicharro, Carmen and Cortes, Sofia and Davidsson, Leigh and Di Muccio, Trentina and Dujardin, Jean-Claude and Felger, Ingrid and Paglia, Maria Grazia and Grimm, Felix and Harms, Gundel and Jaffe, Charles L. and Manser, Monika and Ravel, Christophe and Robert-Gangneux, Florence and Roelfsema, Jeroen and Töz, Seray and Verweij, Jaco J. and Chiodini, Peter L.. (2016) Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014. Eurosurveillance, 21 (49). p. 30418.

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Abstract

Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Molecular Diagnostics (Felger)
UniBasel Contributors:Felger, Ingrid
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1560-7917
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:13 Oct 2017 11:08
Deposited On:20 Apr 2017 12:33

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