Effects of intersyringe processing on adipose tissue and its cellular components: implications in autologous fat grafting

Osinga, Rik and Menzi, Nadia R. and Tchang, Laurent A. H. and Caviezel, Daniel and Kalbermatten, Daniel F. and Martin, Ivan and Schaefer, Dirk J. and Scherberich, Arnaud and Largo, Rene D.. (2015) Effects of intersyringe processing on adipose tissue and its cellular components: implications in autologous fat grafting. Plastic and Reconstructive Surgery, 135 (6). pp. 1618-1628.

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Official URL: http://edoc.unibas.ch/53083/

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Autologous fat grafting is a popular technique in plastic surgery. A mechanical processing method is used to facilitate fat injection. No study has investigated whether this process affects cell quality and preservation of biological functionality. This study analyzed the influence of quick mechanical processing through two interconnected small-diameter syringes ("shuffling") on both structure and viability of fat tissue, and on viability, clonogenicity, and differentiation of the freshly isolated stromal vascular fraction.; Lipoaspiration was performed in six healthy donors, followed by shuffling the fat either zero, five, or 30 times between two 10-cc syringes. Thereafter, fat was applied through a 1.5-mm cannula as in a clinical setting for autologous fat grafting. Analysis of different treatment conditions was conducted. Immunofluorescent staining allowed assessment of morphology, viability, composition, and damage of the tissue. The stromal vascular fraction was examined for isolation yield, viability, clonogenicity, and differentiation capacity.; The process of shuffling changed the macroscopic but not the microscopic structure of the lipoaspirated fat. No difference in cell number, viability, number of lipid droplets, vascular architecture, or ratio of cell composition was found. Analysis of the stromal vascular fraction, apart from large interdonor variability, did not show a significant change in isolation yield, viability, clonogenicity, or adipogenic differentiation capacity of the expanded cells.; The mechanical procedure of shuffling lipoaspirated fat does not alter its tissue viability or its microscopic structure. The absence of impact on the stromal vascular fraction in the assessed parameters suggests that shuffling can be executed according to surgical needs.
Faculties and Departments:03 Faculty of Medicine
UniBasel Contributors:Scherberich, Arnaud
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Lippincott, Williams & Wilkins
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Jun 2018 07:38
Deposited On:04 Oct 2017 15:32

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