Heat shock protein (hsp70) in brown trout epidermis after sudden temperature rise

Burkhardt-Holm, P. and Schmidt, H. and Meier, W.. (1998) Heat shock protein (hsp70) in brown trout epidermis after sudden temperature rise. Comparative Biochemistry and Physiology A, 120 (1). pp. 35-41.

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So far, hsp70 has not yet been studied in the fish skin. This organ has a potential as an indicator organ and we investigate whether hsp70 could be used as a biomarker. In this study, we examined whether and how the epidermis reacts to a temperature rise . Brown trout , Salmo trutta fario, were exposed to higher temperature for 2 h and were allowed to recover subsequently. Samples were taken from controls, after heat shock , as well as after 24 and 48 h of recovery. The occurrence of hsp70 in trout skin was examined by Western blot. The amount of hsp70 was higher after 2-h heat shock and was rising until the end of the experiment. Immunocytochemically, hsp70 was detected in epidermal filament cells. After 2-h heat shock , hsp70 was predominantly located in the nucleus. At this lime, light and electron microscopy revealed several features known to occur under a variety of stressors. Ultrastructurally, the appearance of compact filament aggregates in pavement cells was remarkable. After 24 h of recovery, filament compaction was lacking and after 48 h aspects of regeneration were obvious. However, an increased amount of apoptotic cells in the epidermis was prominent at this time only.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Aquatische ├ľkologie (Holm)
UniBasel Contributors:Holm, Patricia
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Nov 2017 10:20
Deposited On:22 Nov 2017 10:20

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