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Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum

Lamche, G. and Meier, W. and Suter, M. and Burkhardt-Holm, P.. (1998) Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. Cellular and Molecular Life Sciences, 54 (9). pp. 1042-1051.

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Official URL: http://edoc.unibas.ch/52987/

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Abstract

This is the first report on a primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. These primary cells revealed a low seeding efficiency after 3 days (11.6 ± 4.6%), whereas subcultured cells had a higher seeding efficiency at the same time point (75.5 ± 34.0%) and increased in cell number (150 – 200% of seeded cells after 20 to 30 days). The cells were characterized applying histological, immunocytochemical and ultrastructural methods. The culture consisted of undifferentiated keratinocytes. Mucous cells as well as differentiated epithelial cells were absent. To date the cells were cultured for maximally 9 passages and 402 days and therefore provide the possibility for long-term studies.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Mensch Gesellschaft Umwelt MGU > Aquatische Ökologie (Holm)
UniBasel Contributors:Holm, Patricia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Birkhäuser
ISSN:1420-682X
e-ISSN:1420-9071
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:22 Nov 2017 10:28
Deposited On:22 Nov 2017 10:28

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