Unravelling the mechanisms involved in zearalenone-mediated toxicity in permanent fish cell cultures

Pietsch, Constanze and Noser, Jürg and Wettstein, Felix E. and Burkhardt-Holm, Patricia. (2014) Unravelling the mechanisms involved in zearalenone-mediated toxicity in permanent fish cell cultures. Toxicon, 88. pp. 44-61.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/52952/

Downloads: Statistics Overview


The world-wide occurrence of zearalenone (ZEN) as a contaminant in feed for farm animals and fish requires the evaluation of toxicity mechanisms of action of ZEN. The present study investigates possible metabolization of ZEN in fish cell lines suggesting that mainly glucuronidation takes place. It demonstrates that concentrations up to 20,000 ng ml(-1) ZEN are capable of influencing cell viability in permanent fish cell cultures in a dose-response manner with different response patterns between the five tested cell lines, whereby lysosomes appeared to be the main target of ZEN. ZEN toxicity is often discussed in the context of oxidative stress. Our study shows a biphasic response of the cell lines when reactive oxygen species (ROS) production is monitored. Damage in cells was observed by measuring lipid peroxidation, DNA strand breaks, and alterations of intracellular glutathione levels. Metabolization of ZEN, especially at concentrations above 7500 ng ml(-1) ZEN, does not prevent cytotoxicity. ZEN as an estrogenic compound may involve processes mediated by binding to estrogen receptors (ER). Since one cell line showed no detectable expression of ER, an ER-mediated pathway seems to be unlikely in these cells. This confirms a lysosomal pathway as a main target of ZEN in fish cells.
Faculties and Departments:05 Faculty of Science
UniBasel Contributors:Holm, Patricia
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:25 Apr 2018 11:15
Deposited On:25 Apr 2018 11:15

Repository Staff Only: item control page