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Field Trial and Molecular Characterization of RNAi-Transgenic Tomato Plants That Exhibit Resistance to Tomato Yellow Leaf Curl Geminivirus

Fuentes, Alejandro and Carlos, Natacha and Ruiz, Yoslaine and Callard, Danay and Sánchez, Yadira and Ochagavía, María Elena and Seguin, Jonathan and Malpica-López, Nachelli and Hohn, Thomas and Lecca, Maria Rita and Pérez, Rosabel and Doreste, Vivian and Rehrauer, Hubert and Farinelli, Laurent and Pujol, Merardo and Pooggin, Mikhail M.. (2016) Field Trial and Molecular Characterization of RNAi-Transgenic Tomato Plants That Exhibit Resistance to Tomato Yellow Leaf Curl Geminivirus. Molecular Plant-Microbe Interactions, 29 (3). pp. 197-209.

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Official URL: http://edoc.unibas.ch/51889/

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Abstract

RNA interference (RNAi) is a widely used approach to generate virus-resistant transgenic crops. However, issues of agricultural importance like the long-term durability of RNAi-mediated resistance under field conditions and the potential side effects provoked in the plant by the stable RNAi expression remain poorly investigated. Here, we performed field trials and molecular characterization studies of two homozygous transgenic tomato lines, with different selection markers, expressing an intron-hairpin RNA cognate to the Tomato yellow leaf curl virus (TYLCV) C1 gene. The tested F6 and F4 progenies of the respective kanamycin- and basta-resistant plants exhibited unchanged field resistance to TYLCV and stably expressed the transgene-derived short interfering RNA (siRNAs) to represent 6 to 8% of the total plant small RNAs. This value outnumbered the average percentage of viral siRNAs in the nontransformed plants exposed to TYLCV-infested whiteflies. As a result of the RNAi transgene expression, a common set of up- and downregulated genes was revealed in the transcriptome profile of the plants selected from either of the two transgenic events. A previously unidentified geminivirus causing no symptoms of viral disease was detected in some of the transgenic plants. The novel virus acquired V1 and V2 genes from TYLCV and C1, C2, C3, and C4 genes from a distantly related geminivirus and, thereby, it could evade the repressive sequence-specific action of transgene-derived siRNAs. Our findings shed light on the mechanisms of siRNA-directed antiviral silencing in transgenic plants and highlight the applicability limitations of this technology as it may alter the transcriptional pattern of nontarget genes.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik > Pflanzenphysiologie Pathogenabwehr (Boller)
UniBasel Contributors:Pooggin, Mikhail and Malpica López, Hortensia Nachelli and Hohn, Thomas and Seguin, Jonathan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Phytopathological Society
ISSN:0894-0282
e-ISSN:1943-7706
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:22 Mar 2018 14:43
Deposited On:26 Oct 2017 13:10

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