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Formation of Actin Networks in Microfluidic Concentration Gradients

Strelnikova, N. and Herren, F. and Schoenenberger, C. A. and Pfohl, T.. (2016) Formation of Actin Networks in Microfluidic Concentration Gradients. Frontiers Materials - biomaterials, 3. p. 20.

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Abstract

The physical properties of cytoskeletal networks are contributors in a number of mechanical responses of cells, including cellular deformation and locomotion, and are crucial for the proper action of living cells. Local chemical gradients modulate cytoskeletal functionality including the interactions of the cytoskeleton with other cellular components. Actin is a major constituent of the cytoskeleton. Introducing a microfluidic-based platform, we explored the impact of concentration gradients on the formation and structural properties of actin networks. Microfluidic-controlled flow-free and steady-state experimental conditions allow for the generation of chemical gradients of different profiles, such as linear or step-like. We discovered specific features of actin networks emerging in defined gradients. In particular, we analyzed the effects of spatial conditions on network properties, bending rigidities of network links, and the network elasticity.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Frontiers Media
e-ISSN:2296-8016
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:23 Feb 2017 15:22
Deposited On:23 Feb 2017 15:22

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