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Biasing effects of receptor-ligand complexes on protein-unfolding statistics

Schoeler, Constantin and Verdorfer, Tobias and Gaub, Hermann E. and Nash, Michael A.. (2016) Biasing effects of receptor-ligand complexes on protein-unfolding statistics. Physical review E, 94 (4). 042412.

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Official URL: http://edoc.unibas.ch/51755/

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Abstract

Protein receptor-ligand pairs are increasingly used as specific molecular handles in single-molecule protein-unfolding experiments. Further, known marker domains, also referred to as fingerprints, provide unique unfolding signatures to identify specific single-molecule interactions, when receptor-ligand pairs themselves are investigated. We show here that in cases where there is an overlap between the probability distribution associated with fingerprint domain unfolding and that associated with receptor-ligand dissociation, the experimentally measured force distributions are mutually biased. This biasing effect masks the true parameters of the underlying free energy landscape. To address this, we present a model-free theoretical framework that corrects for the biasing effect caused by such overlapping distributions.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Synthetic Systems (Nash)
UniBasel Contributors:Nash, Michael
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Physical Society
ISSN:2470-0045
e-ISSN:2470-0053
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:14 Jun 2017 07:50
Deposited On:14 Jun 2017 07:45

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