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Mechanical Stability of a High-Affinity Toxin Anchor from the Pathogen Clostridium perfringens

Milles, Lukas Frederik and Bayer, Edward A. and Nash, Michael A. and Gaub, Hermann E.. (2017) Mechanical Stability of a High-Affinity Toxin Anchor from the Pathogen Clostridium perfringens. Journal of Physical Chemistry B, 121 (15). pp. 3620-3625.

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Official URL: http://edoc.unibas.ch/51752/

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Abstract

The opportunistic pathogen Clostridium perfringens assembles its toxins and carbohydrate-active enzymes by the high-affinity cohesin-dockerin (Coh-Doc) interaction. Coh-Doc interactions characterized previously have shown considerable resilience towards mechanical stress. Here, we aimed to determine the mechanics of this interaction from C. perfringens in the context of a pathogen. Using atomic force microscopy based single-molecule force spectroscopy (AFM-SMFS) we probed the mechanical properties of the interaction of a dockerin from the $mu$-toxin with the GH84C X82 cohesin domain of C. perfringens. Most probable complex rupture forces were found to be approximately 60 pN. An estimate of the binding potential width was performed using two different methods of loading rate determination. The dockerin was expressed with its adjacent FIVAR (Found in Various Architectures) domain, whose mechanostability we determined to be very similar to the complex. Additionally, fast refolding of this domain was observed. The Coh-Doc interaction from C. perfringens is the mechanically weakest observed to date. Our results establish the relevant force range of toxin assembly mechanics in pathogenic Clostridia.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Synthetic Systems (Nash)
UniBasel Contributors:Nash, Michael
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
ISSN:1520-6106
e-ISSN:1520-5207
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:14 Jun 2017 08:01
Deposited On:14 Jun 2017 07:31

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