Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window

Holtmaat, Anthony and Bonhoeffer, Tobias and Chow, David K. and Chuckowree, Jyoti and De Paola, Vincenzo and Hofer, Sonja B. and Hübener, Mark and Keck, Tara and Knott, Graham and Lee, Wei-Chung A. and Mostany, Ricardo and Mrsic-Flogel, Tom D. and Nedivi, Elly and Portera-Cailliau, Carlos and Svoboda, Karel and Trachtenberg, Joshua T. and Wilbrecht, Linda. (2009) Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nature Protocols, 4 (8). pp. 1128-1144.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/49619/

Downloads: Statistics Overview


To understand the cellular and circuit mechanisms of experience-dependent plasticity, neurons and their synapses need to be studied in the intact brain over extended periods of time. Two-photon excitation laser scanning microscopy (2PLSM), together with expression of fluorescent proteins, enables high-resolution imaging of neuronal structure in vivo. In this protocol we describe a chronic cranial window to obtain optical access to the mouse cerebral cortex for long-term imaging. A small bone flap is replaced with a coverglass, which is permanently sealed in place with dental acrylic, providing a clear imaging window with a large field of view (approximately 0.8-12 mm(2)). The surgical procedure can be completed within approximately 1 h. The preparation allows imaging over time periods of months with arbitrary imaging intervals. The large size of the imaging window facilitates imaging of ongoing structural plasticity of small neuronal structures in mice, with low densities of labeled neurons. The entire dendritic and axonal arbor of individual neurons can be reconstructed.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Neuronal circuits and brain function (Hofer)
UniBasel Contributors:Hofer, Sonja and Mrsic-Flogel, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:29 Nov 2017 08:53
Deposited On:29 Nov 2017 08:53

Repository Staff Only: item control page