Cre-dependent expression of multiple transgenes in isolated neurons of the adult forebrain

Chakravarthy, Sridhara and Keck, Tara and Roelandse, Martijn and Hartman, Robin and Jeromin, Andreas and Perry, Sean and Hofer, Sonja B. and Mrsic-Flogel, Thomas and Levelt, Christiaan N.. (2008) Cre-dependent expression of multiple transgenes in isolated neurons of the adult forebrain. PLoS ONE, 3 (8). e3059.

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Official URL: http://edoc.unibas.ch/49616/

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Transgenic mice with mosaic, Golgi-staining-like expression of enhanced green fluorescent protein (EGFP) have been very useful in studying the dynamics of neuronal structure and function. In order to further investigate the molecular events regulating structural plasticity, it would be useful to express multiple proteins in the same sparse neurons, allowing co-expression of functional proteins or co-labeling of subcellular compartments with other fluorescent proteins. However, it has been difficult to obtain reproducible expression in the same subset of neurons for direct comparison of neurons expressing different functional proteins.; Here we describe a Cre-transgenic line that allows reproducible expression of transgenic proteins of choice in a small number of neurons of the adult cortex, hippocampus, striatum, olfactory bulb, subiculum, hypothalamus, superior colliculus and amygdala. We show that using these Cre-transgenic mice, multiple Cre-dependent transgenes can be expressed together in the same isolated neurons. We also describe a Cre-dependent transgenic line expressing a membrane associated EGFP (EGFP-F). Crossed with the Cre-transgenic line, EGFP-F expression starts in the adolescent forebrain, is present in dendrites, dendritic protrusions, axons and boutons and is strong enough for acute or chronic in vivo imaging.; This triple transgenic approach will aid the morphological and functional characterization of neurons in various Cre-dependent transgenic mice.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Neuronal circuits and brain function (Hofer)
UniBasel Contributors:Hofer, Sonja and Mrsic-Flogel, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Public Library of Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Aug 2018 06:33
Deposited On:28 Nov 2017 10:36

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