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High throughput multiplex assay for species identification of Papua New Guinea malaria vectors : members of the Anopheles punctulatus (Diptera: Culicidae) species group

Henry-Halldin, Cara N. and Reimer, Lisa and Thomsen, Edward and Koimbu, Gussy and Zimmerman, Allison and Keven, John B. and Dagoro, Henry and Hetzel, Manuel W. and Mueller, Ivo and Siba, Peter and Zimmerman, Peter A.. (2011) High throughput multiplex assay for species identification of Papua New Guinea malaria vectors : members of the Anopheles punctulatus (Diptera: Culicidae) species group. American journal of tropical medicine and hygiene, 84 (1). pp. 166-173.

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Official URL: http://edoc.unibas.ch/49568/

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Abstract

Malaria and filariasis are transmitted in the Southwest Pacific region by Anopheles punctulatus sibling species including An. punctulatus, An. koliensis, the An. farauti complex 1-8 (includes An. hinesorum [An. farauti 2], An. torresiensis [An. farauti 3]). Distinguishing these species from each other requires molecular diagnostic methods. We developed a multiplex polymerase chain reaction (PCR)-based assay specific for known species-specific nucleotide differences in the internal transcribed spacer 2 region and identified the five species most frequently implicated in transmitting disease (An. punctulatus, An. koliensis, An. farauti 1, An. hinesorum, and An. farauti 4). A set of 340 individual mosquitoes obtained from seven Papua New Guinea provinces representing a variety of habitats were analyzed by using this multiplex assay. Concordance between molecular and morphological diagnosis was 56.4% for An. punctulatus, 85.3% for An. koliensis, and 88.9% for An. farauti. Among 158 mosquitoes morphologically designated as An. farauti, 33 were re-classified by PCR as An. punctulatus, 4 as An. koliensis, 26 as An. farauti 1, 49 as An. hinesorum, and 46 as An. farauti 4. Misclassification results from variable coloration of the proboscis and overlap of An. punctulatus, An. koliensis, the An. farauti 4. This multiplex technology enables further mosquito strain identification and simultaneous detection of microbial pathogens.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Health Interventions > Malaria Interventions (Lengeler)
UniBasel Contributors:Hetzel, Manuel W
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Williams and Wilkins
ISSN:0002-9637
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:04 Apr 2017 12:37
Deposited On:04 Apr 2017 12:37

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