Comprehensive description of the N-glycoproteome of mouse pancreatic ?-cells and human islets

Danzer, Carsten and Eckhardt, Katrin and Schmidt, Alexander and Fankhauser, Niklaus and Ribrioux, Sebastien and Wollscheid, Bernd and Müller, Lukas and Schiess, Ralph and Züllig, Richard and Lehmann, Roger and Spinas, Giatgen and Aebersold, Rudolf and Krek, Wilhelm. (2012) Comprehensive description of the N-glycoproteome of mouse pancreatic ?-cells and human islets. Journal of Proteome Research, 11 (3). pp. 1598-1608.

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Cell surface N-glycoproteins provide a key interface of cells to their environment and therapeutic entry points for drug and biomarker discovery. Their comprehensive description denotes therefore a formidable challenge. The ?-cells of the pancreas play a crucial role in blood glucose homeostasis, and disruption of their function contributes to diabetes. By combining cell surface and whole cell capturing technologies with high-throughput quantitative proteomic analysis, we report on the identification of a total of 956 unique N-glycoproteins from mouse MIN6 ?-cells and human islets. Three-hundred-forty-nine of these proteins encompass potential surface N-glycoproteins and include orphan G-protein-coupled receptors, novel proteases, receptor protein kinases, and phosphatases. Interestingly, stimulation of MIN6 ?-cells with glucose and the hormone GLP1, known stimulators of insulin secretion, causes significant changes in surface N-glycoproteome expression. Taken together, this ?-cell N-glycoproteome resource provides a comprehensive view on the composition of ?-cell surface proteins and expands the scope of signaling systems potentially involved in mediating responses of ?-cells to various forms of (patho)physiologic stress and the extent of dynamic remodeling of surface N-glycoprotein expression associated with metabolic and hormonal stimulation. Moreover, it provides a foundation for the development of diabetes medicines that target or are derived from the ?-cell surface N-glycoproteome.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:30 Nov 2017 10:54
Deposited On:30 Nov 2017 10:54

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