Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection

Carinhas, N. and Robitaille, A. M. and Moes, S. and Carrondo, M. J. and Jenoe, P. and Oliveira, R. and Alves, P. M.. (2011) Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. PLoS ONE, 6 (10). e26444.

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Official URL: http://edoc.unibas.ch/46903/

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Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Mass Spectrometry (Jenö)
UniBasel Contributors:Jenö, Paul and Robitaille, Aaron Mark and Moes, Suzanne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Public Library of Science
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:31 Aug 2018 06:33
Deposited On:18 Jan 2018 10:10

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