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A 28-bp segment of the Saccharomyces cerevisiae PHO5 upstream activator sequence confers phosphate control to the CYC1-lacZ gene fusion

Sengstag, Christian and Hinnen, Albert. (1988) A 28-bp segment of the Saccharomyces cerevisiae PHO5 upstream activator sequence confers phosphate control to the CYC1-lacZ gene fusion. Gene, 67 (2). pp. 223-228.

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Official URL: http://edoc.unibas.ch/46836/

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Abstract

Two regions within the Saccharomyces cerevisiae PHO5 upstream activator sequence (UAS) are involved in phosphate dependent transcription activation [Rudolph and Hinnen, Proc. Natl. Acad. Sci. USA 84 (1987) 1340-1344]. In experiments carried out in vivo we showed that one of these can compensate for the CYC1 UAS and expresses the heterologous CYC1-lacZ gene in response to phosphate starvation. A 28-bp segment is very efficient in gene activation, and a 19-bp subsegment that corresponds to the UASp consensus sequence brings about a weak but still detectable activation. As was observed with other UAS, gene activation is obtained with either orientation of the element, and tandem copies yield double lacZ activity compared to a single copy. No gene activation is observed in a pho4 and in a pho2 mutant. Absence of PHO2 reduces the basal expression of CYC1.
Faculties and Departments:11 Rektorat und Verwaltung > Vizerektorat Forschung
UniBasel Contributors:Sengstag, Christian
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0378-1119
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Nov 2021 13:08
Deposited On:24 Nov 2021 13:08

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