Raman and surface enhanced Raman microscopy of microstructured PEI/DNA multilayers

Dootz, Rolf and Nie, Jingjing and Du, Binyang and Herminghaus, Stephan and Pfohl, Thomas. (2006) Raman and surface enhanced Raman microscopy of microstructured PEI/DNA multilayers. Langmuir, 22 (4). pp. 1735-1741.

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Official URL: http://edoc.unibas.ch/46504/

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We analyze microstructured multilayer films of poly(ethyleneimine) (PEI) and DNA by employing Raman and surface enhanced Raman spectroscopy (SERS). The microstructuring of the samples allows a simultaneous measurement of signal and reference in a single analytic process. Silver nanoparticles are implemented in the microstructured multilayers for SERS measurements. The recorded SERS spectra of PEI/DNA are dominated by the Raman bands of the DNA bases which show a larger mean enhancement than bands belonging to DNA backbone vibrations. Our results show that the combination of SERS and microstructured multilayer films provides an adapted way to characterize the polyelectrolytes as well as to measure the enhancement factor and the distance dependence for the SERS active silver nanoparticles. Furthermore, microstructured polyelectrolyte films containing SERS active nanoparticles are used for sensing molecules.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Former Organization Units Chemistry > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:19 Dec 2016 14:38
Deposited On:19 Dec 2016 14:38

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