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A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization

Ozbudak, Ertugrul M. and Becskei, Attila and van Oudenaarden, Alexander. (2005) A system of counteracting feedback loops regulates Cdc42p activity during spontaneous cell polarization. Developmental Cell, 9 (4). pp. 565-571.

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Official URL: http://edoc.unibas.ch/46411/

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Abstract

Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.
Faculties and Departments:05 Faculty of Science
05 Faculty of Science > Departement Biozentrum
05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Synthetic Microbiology (Becskei)
UniBasel Contributors:Becskei, Attila
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1534-5807
e-ISSN:1878-1551
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:23 Nov 2017 08:12
Deposited On:23 Nov 2017 08:12

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