Life-style changes of a halophilic archaeon analyzed by quantitative proteomics

Tebbe, Andreas and Schmidt, Alexander and Konstantinidis, Kosta and Falb, Michaela and Bisle, Birgit and Klein, Christian and Aivaliotis, Michalis and Kellermann, Josef and Siedler, Frank and Pfeiffer, Friedhelm and Lottspeich, Friedrich and Oesterhelt, Dieter . (2009) Life-style changes of a halophilic archaeon analyzed by quantitative proteomics. Proteomics, 9 (15). pp. 3843-3855.

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Official URL: http://edoc.unibas.ch/45904/

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Quantitative proteomics based on isotopic labeling has become the method of choice to accurately determine changes in protein abundance in highly complex mixtures. Isotope-coded protein labeling (ICPL), which is based on the nicotinoylation of proteins at lysine residues and free N-termini was used as a simple, reliable and fast method for the comparative analysis of three different cellular states of the halophilic archaeon Halobacterium salinarum through pairwise comparison. The labeled proteins were subjected to SDS-PAGE, in-gel digested and the proteolytic peptides were separated by LC and analyzed by MALDI-TOF/TOF MS. Automated quantitation was performed by comparing the MS peptide signals of (12)C and (13)C nicotinoylated isotopic peptide pairs. The transitions between (i) aerobic growth in complex versus synthetic medium and (ii) aerobic versus anaerobic/phototrophic growth, both in complex medium, provide a wide span in nutrient and energy supply for the cell and thus allowed optimal studies of proteome changes. In these two studies, 559 and 643 proteins, respectively, could be quantified allowing a detailed analysis of the adaptation of H. salinarum to changes of its living conditions. The subtle cellular response to a wide variation of nutrient and energy supply demonstrates a fine tuning of the cellular protein inventory.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:29 Nov 2017 09:17
Deposited On:29 Nov 2017 09:17

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