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Highly packed and oriented DNA mesophases identified using in situ microfluidic X-ray microdiffraction

Pfohl, Thomas and Otten, Alexander and Köster, Sarah and Dootz, Rolf and Struth, Bernd and Evans, Heather M.. (2007) Highly packed and oriented DNA mesophases identified using in situ microfluidic X-ray microdiffraction. Biomacromolecules, 8 (7). pp. 2167-2172.

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Official URL: http://edoc.unibas.ch/45896/

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Abstract

DNA condensation in vivo usually requires proteins and/or multivalent salts. Here, we explore the in vitro compaction of DNA by cationic dendrimers having an intermediate size and charge. The dynamic assembly of DNA-dendrimer mesophases is discernible due to the laminar flow in a specially designed X-ray compatible microfluidic device. The setup ensures a nonequilibrium ascent of reactant concentration, and the resulting progression of DNA compaction was detected online using microfocused small-angle X-ray diffraction. The evolution of a DNA-dendrimer columnar square mesophase as a function of increasing dendrimer content is described. Additionally, in regions of maximum shear, an unexpected high-level perpendicular ordering of this phase is recorded. Furthermore, these assemblies are found to be in coexistence with a densely packed DNA-only mesophase in regions of excess DNA. The latter is reminiscent of dense packing found in bacteriophage and chromosomes, although surprisingly, it is not stabilized by direct dendrimer contact.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
ISSN:1525-7797
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Dec 2016 13:38
Deposited On:19 Dec 2016 13:38

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