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Inhibition of Tobacco mosaic virus movement by expression of an actin-binding protein

Hofmann, Christina and Niehl, Annette and Sambade, Adrian and Steinmetz, André and Heinlein, Manfred. (2009) Inhibition of Tobacco mosaic virus movement by expression of an actin-binding protein. Plant Physiology , 149 (4). pp. 1810-1823.

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Official URL: http://edoc.unibas.ch/45444/

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Abstract

The tobacco mosaic virus (TMV) movement protein (MP) required for the cell-to-cell spread of viral RNA interacts with the endoplasmic reticulum (ER) as well as with the cytoskeleton during infection. Whereas associations of MP with ER and microtubules have been intensely investigated, research on the role of actin has been rather scarce. We demonstrate that Nicotiana benthamiana plants transgenic for the actin-binding domain 2 of Arabidopsis (Arabidopsis thaliana) fimbrin (AtFIM1) fused to green fluorescent protein (ABD2:GFP) exhibit a dynamic ABD2:GFP-labeled actin cytoskeleton and myosin-dependent Golgi trafficking. These plants also support the movement of TMV. In contrast, both myosin-dependent Golgi trafficking and TMV movement are dominantly inhibited when ABD2:GFP is expressed transiently. Inhibition is mediated through binding of ABD2:GFP to actin filaments, since TMV movement is restored upon disruption of the ABD2:GFP-labeled actin network with latrunculin B. Latrunculin B shows no significant effect on the spread of TMV infection in either wild-type plants or ABD2:GFP transgenic plants under our treatment conditions. We did not observe any binding of MP along the length of actin filaments. Collectively, these observations demonstrate that TMV movement does not require an intact actomyosin system. Nevertheless, actin-binding proteins appear to have the potential to exert control over TMV movement through the inhibition of myosinassociated protein trafficking along the ER membrane.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik
UniBasel Contributors:Heinlein, Manfred
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society of Plant Biologists
ISSN:0032-0889
e-ISSN:1532-2548
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Jan 2018 08:50
Deposited On:12 Jan 2018 08:50

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