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Tobacco mosaic virus movement protein interacts with green fluorescent protein-tagged microtubule end-binding protein 1

Brandner, Katrin and Sambade, Adrian and Boutant, Emmanuel and Didier, Pascal and Mély, Yves and Ritzenthaler, Chistophe and Heinlein, Manfred. (2008) Tobacco mosaic virus movement protein interacts with green fluorescent protein-tagged microtubule end-binding protein 1. Plant Physiology , 147 (2). pp. 611-623.

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Official URL: http://edoc.unibas.ch/45441/

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Abstract

The targeting of the movement protein (MP) of Tobacco mosaic virus to plasmodesmata involves the actin/endoplasmic reticulum network and does not require an intact microtubule cytoskeleton. Nevertheless, the ability ofMPto facilitate the cell-to-cell spread of infection is tightly correlated with interactions of the protein with microtubules, indicating that the microtubule system is involved in the transport of viral RNA. While the MP acts like a microtubule-associated protein able to stabilize microtubules during late infection stages, the protein was also shown to cause the inactivation of the centrosome upon expression in mammalian cells, thus suggesting that MP may interact with factors involved in microtubule attachment, nucleation, or polymerization. To further investigate the interactions of MP with the microtubule system in planta, we expressed the MP in the presence of green fluorescent protein (GFP)-fused microtubule end-binding protein 1a (EB1a) of Arabidopsis (Arabidopsis thaliana; AtEB1a:GFP). The two proteins colocalize and interact in vivo as well as in vitro and exhibit mutual functional interference. These findings suggest thatMPinteracts with EB1 and that this interaction may play a role in the associations ofMPwith the microtubule system during infection.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Botanik
UniBasel Contributors:Heinlein, Manfred
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Society of Plant Biologists
ISSN:0032-0889
e-ISSN:1532-2548
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Dec 2017 11:04
Deposited On:19 Dec 2017 11:04

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