Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

Bauer, Manuel and Cubizolles, Fabien and Schmidt, Alexander and Nigg, Erich A.. (2016) Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging. The EMBO journal, 35 (19). pp. 2152-2166.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/44083/

Downloads: Statistics Overview


Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly, and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas-6, and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes is presently known. Here, we have used two complementary approaches, targeted proteomics and EGFP-tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. Specifically, they predict that human centriolar cartwheels comprise up to 16 stacked hubs and 1 molecule of STIL for every dimer of Sas-6. This type of quantitative information will help guide future studies of the molecular basis of centrosome assembly and function.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A. and Cubizolles, Fabien and Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:EMBO Press
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:25 Oct 2017 12:29
Deposited On:25 Oct 2017 12:29

Repository Staff Only: item control page