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Mechanical control of mitotic progression in single animal cells

Cattin, Cedric J. and Düggelin, Marcel and Martinez-Martin, David and Gerber, Christoph and Müller, Daniel J. and Stewart, Martin P.. (2015) Mechanical control of mitotic progression in single animal cells. Proceedings of the National Academy of Sciences of the United States of America, 112 (36). pp. 11258-11263.

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Official URL: http://edoc.unibas.ch/44023/

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Abstract

Despite the importance of mitotic cell rounding in tissue development and cell proliferation, there remains a paucity of approaches to investigate the mechanical robustness of cell rounding. Here we introduce ion beam-sculpted microcantilevers that enable precise force-feedback-controlled confinement of single cells while characterizing their progression through mitosis. We identify three force regimes according to the cell response: small forces (∼5 nN) that accelerate mitotic progression, intermediate forces where cells resist confinement (50-100 nN), and yield forces (>100 nN) where a significant decline in cell height impinges on microtubule spindle function, thereby inhibiting mitotic progression. Yield forces are coincident with a nonlinear drop in cell height potentiated by persistent blebbing and loss of cortical F-actin homogeneity. Our results suggest that a buildup of actomyosin-dependent cortical tension and intracellular pressure precedes mechanical failure, or herniation, of the cell cortex at the yield force. Thus, we reveal how the mechanical properties of mitotic cells and their response to external forces are linked to mitotic progression under conditions of mechanical confinement.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik
UniBasel Contributors:Gerber, Christoph
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:National Academy of Sciences
ISSN:0027-8424
e-ISSN:1091-6490
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:08 Feb 2019 15:35
Deposited On:31 Aug 2016 09:24

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