Development and full validation of an UPLC-MS/MS method for the quantification of indirubin in rat plasma

Jähne, Evelyn A. and Sampath, Chethan and Butterweck, Veronika and Hamburger, Matthias and Oufir, Mouhssin. (2016) Development and full validation of an UPLC-MS/MS method for the quantification of indirubin in rat plasma. Journal of Pharmaceutical and Biomedical Analysis, 128. pp. 247-252.

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An UPLC-MS/MS method for the quantification of indirubin in lithium heparinized rat plasma was developed and validated according to current international guidelines. Indirubin was extracted from rat plasma by using Waters Ostro™ pass-through sample preparation plates. The method was validated with a LLOQ of 5.00 ng/mL and an ULOQ of 500 ng/mL. The calibration curve was fitted by least-square quadratic regression, and a weighting factor of 1/X was applied. Recoveries of indirubin and I.S. were consistent and ≥75.5%. Stability studies demonstrated that indirubin was stable in lithium heparinized rat plasma for at least 3 freeze/thaw cycles, for 3 h at RT, for 96 h in the autosampler at 10 °C, and for 84 days when stored below −65 °C. Preliminary pharmacokinetic (PK) data were obtained from Sprague Dawley rats after intravenous administration of indirubin (2 mg/kg b.w.) and blood sampling up to 12 h after injection. PK parameters were determined by non-compartmental analysis. Indirubin had a half-life (t1/2) of 35 min, and a relatively high clearance (CL) of 2.71 L/h/kg.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Ehemalige Einheiten Pharmazie > Pharmazeutische Biologie (Hamburger)
UniBasel Contributors:Hamburger, Matthias and Jähne, Evelyn and Oufir, Mouhssin
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Nov 2017 09:58
Deposited On:13 Dec 2016 09:17

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