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Validation of a point-of-care circulating cathodic antigen urine cassette test for Schistosoma mansoni diagnosis in the Sahel, and potential cross-reaction in pregnancy

Greter, Helena and Krauth, Stefanie J. and Ngandolo, Bongo N. R. and Alfaroukh, Idriss O. and Zinsstag, Jakob and Utzinger, Jürg. (2016) Validation of a point-of-care circulating cathodic antigen urine cassette test for Schistosoma mansoni diagnosis in the Sahel, and potential cross-reaction in pregnancy. American journal of tropical medicine and hygiene, 94 (2). pp. 361-364.

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Official URL: http://edoc.unibas.ch/42135/

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Abstract

On the shores of Lake Chad, schistosomiasis among mobile pastoralists was investigated in a field laboratory. Point-of-care circulating cathodic antigen (POC-CCA) cassette test, reagent strip, and filtration were conducted on urine samples. Fresh stool samples were subjected to the Kato-Katz technique, and fixed samples were examined with an ether-concentration method at a reference laboratory. POC-CCA urine cassette tests revealed a Schistosoma mansoni prevalence of 6.9%, compared with only 0.5% by stool microscopy. Three pregnant women with otherwise negative urine and stool testing had positive POC-CCA. This observation raises concern of cross-reactivity in pregnancy. Hence, two pregnant women in Switzerland with no history of schistosomiasis were subjected to POC-CCA and one tested positive. Our data suggest that POC-CCA can be performed under extreme Sahelian conditions (e.g., temperatures > 40°C), and it is more sensitive than stool microscopy for S. mansoni diagnosis. However, potential cross-reactivity in pregnancy needs further investigation.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Health Impact Assessment (Utzinger)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Human and Animal Health > One Health (Zinsstag)
UniBasel Contributors:Greter, Helena and Zinsstag, Jakob Z and Utzinger, Jürg
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Williams and Wilkins
ISSN:0002-9637
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:30 Jun 2016 11:03
Deposited On:28 Apr 2016 08:13

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