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Microfluidics-based single cell analysis reveals drug-dependent motility changes in trypanosomes

Hochstetter, Axel and Stellamanns, Eric and Deshpande, Siddharth and Uppaluri, Sravanti and Engstler, Markus and Pfohl, Thomas. (2015) Microfluidics-based single cell analysis reveals drug-dependent motility changes in trypanosomes. Lab on a chip, 15 (8). pp. 1961-1968.

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Official URL: http://edoc.unibas.ch/41485/

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Abstract

We present a single cell viability assay, based on chemical gradient microfluidics in combination with optical micromanipulation. Here, we used this combination to in situ monitor the effects of drugs and chemicals on the motility of the flagellated unicellular parasite Trypanosoma brucei; specifically, the local cell velocity and the mean squared displacement (MSD) of the cell trajectories. With our method, we are able to record in situ cell fixation by glutaraldehyde, and to quantify the critical concentration of 2-deoxy-d-glucose required to completely paralyze trypanosomes. In addition, we detected and quantified the impact on cell propulsion and energy generation at much lower 2-deoxy-d-glucose concentrations. Our microfluidics-based approach advances fast cell-based drug testing in a way that allows us to distinguish cytocidal from cytostatic drug effects, screen effective dosages, and investigate the impact on cell motility of drugs and chemicals. Using suramin, we could reveal the impact of the widely used drug on trypanosomes: suramin lowers trypanosome motility and induces cell-lysis after endocytosis.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Royal Society of Chemistry
ISSN:1473-0189
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:02 Jul 2019 08:06
Deposited On:18 May 2016 11:53

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