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Polycomb function during oogenesis is essential for mouse embryonic development

Posfai, Eszter and Kunzmann, Rico and Brochard, Vincent and Salvaing, Juliette and Cabuy, Erik and Roloff, Tim C. and Liu, Zichuan and Tardat, Mathieu and van Lohuizen, Maarten and Vidal, Miguel and Beaujean, Nathalie and Peters, Antoine H. F. M.. (2012) Polycomb function during oogenesis is essential for mouse embryonic development. Genes & development, 26 (9). pp. 920-932.

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Official URL: http://edoc.unibas.ch/40113/

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Abstract

In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei in reproductive cloning suggests that intergenerational inheritance of germline chromatin contributes to developmental proficiency after natural conception. Here we show that Ring1 and Rnf2 , components of Polycomb-repressive complex 1 (PRC1), serve redundant transcriptional functions during oogenesis that are essential for proper ZGA, replication and cell cycle progression in early embryos, and development beyond the two-cell stage. Exchange of chromosomes between control and Ring1 / Rnf2 -deficient metaphase II oocytes reveal cytoplasmic and chromosome-based contributions by PRC1 to embryonic development. Our results strongly support a model in which Polycomb acts in the female germline to establish developmental competence for the following generation by silencing differentiation-inducing genes and defining appropriate chromatin states.
Faculties and Departments:09 Associated Institutions > Friedrich Miescher Institut FMI > Epigenetics > Epigenetic control of mouse germ cell and early embryonic development (Peters)
UniBasel Contributors:Peters, Antoine
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Cold Spring Harbor Laboratory
ISSN:0890-9369
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:30 Jun 2016 11:00
Deposited On:29 Jan 2016 09:05

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