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Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I

Stettler, Alexandra R. and Chaurin, Valerie and Constable, Edwin C. and Housecroft, Catherine E. and Schwarz, Maria A.. (2007) Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I. Journal of Biological Inorganic Chemistry, 12 (2). pp. 194-203.

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Official URL: http://edoc.unibas.ch/39167/

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Abstract

The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Anorganische Chemie (Constable)
UniBasel Contributors:Constable, Edwin Charles
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer
ISSN:0949-8257
e-ISSN:1432-1327
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Apr 2017 07:17
Deposited On:26 Jan 2016 08:07

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