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Filling Polymersomes with Polymers by Peroxidase-Catalyzed Atom Transfer Radical Polymerization

Dinu, Maria Valentina and Spulber, Mariana and Renggli, Kasper and Wu, Dalin and Monnier, Christophe A. and Petri-Fink, Alke and Bruns, Nico. (2015) Filling Polymersomes with Polymers by Peroxidase-Catalyzed Atom Transfer Radical Polymerization. Macromolecular rapid communications, Vol. 36, H. 6. pp. 507-514.

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Official URL: http://edoc.unibas.ch/dok/A6419747

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Abstract

Polymersomes that encapsulate a hydrophilic polymer are prepared by conducting biocatalytic atom transfer radical polymerization (ATRP) in these hollow nanostructures. To this end, ATRPase horseradish peroxidase (HRP) is encapsulated into vesicles self-assembled from poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) diblock copolymers. The vesicles are turned into nanoreactors by UV-induced permeabilization with a hydroxyalkyl phenone and used to polymerize poly(ethylene glycol) methyl ether acrylate (PEGA) by enzyme-catalyzed ATRP. As the membrane of the polymersomes is only permeable for the reagents of ATRP but not for macromolecules, the polymerization occurs inside of the vesicles and fills the polymersomes with poly(PEGA), as evidenced by 1H NMR. Dynamic and static light scattering show that the vesicles transform from hollow spheres to filled spheres during polymerization. Transmission electron microscopy (TEM) and cryo-TEM imaging reveal that the polymersomes are stable under the reaction conditions. The polymer-filled nanoreactors mimic the membrane and cytosol of cells and can be useful tools to study enzymatic behavior in crowded macromolecular environments.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie
05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)
UniBasel Contributors:Bruns, Nico and Dinu, Maria Valentina and Spulber, Mariana and Renggli, Kasper and Wu, Dalin
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Wiley-VCH
ISSN:1022-1336
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:02 Oct 2015 10:00
Deposited On:02 Oct 2015 10:00

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