Electron and atomic force microscopy of the trimeric ammonium transporter AmtB

Conroy, M. J. and Jamieson, S. J. and Blakey, D. and Kaufmann, T. and Engel, A. and Fotiadis, D. and Merrick, M. and Bullough, P. A.. (2004) Electron and atomic force microscopy of the trimeric ammonium transporter AmtB. EMBO reports, Vol. 5, H. 12. pp. 1153-1158.

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Official URL: http://edoc.unibas.ch/dok/A5262403

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Escherichia coli AmtB is an archetypal member of the ammonium transporter (Amt) family, a family of proteins that are conserved in all domains of life. Reconstitution of AmtB in the presence of lipids produced large, ordered two-dimensional crystals. From these, a 12 A resolution projection map was determined by cryoelectron microscopy, and high-resolution topographs were acquired using atomic force microscopy. Both techniques showed the trimeric structure of AmtB in which each monomer seems to have a pseudo-two-fold symmetry. This arrangement is likely to represent the in vivo structure. This work provides the first views of the structure of any member of the Amt family.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Engel)
UniBasel Contributors:Engel, Andreas H
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:23

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