Neutrophil elastase-mediated increase in airway temperature during inflammation

Schmidt, Annika and Belaaouaj, Azzaq and Bissinger, Rosi and Koller, Garrit and Malleret, Laurette and D'Orazio, Ciro and Facchinelli, Martino and Schulte-Hubbert, Bernhard and Molinaro, Antonio and Holst, Otto and Hammermann, Jutta and Schniederjans, Monika and Meyer, Keith C. and Damkiaer, Soeren and Piacentini, Giorgio and Assael, Baroukh and Bruce, Kenneth and Häußler, Susanne and LiPuma, John J. and Seelig, Joachim and Worlitzsch, Dieter and Döring, Gerd. (2014) Neutrophil elastase-mediated increase in airway temperature during inflammation. Journal of Cystic Fibrosis, 13 (6). pp. 623-631.

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Official URL: http://edoc.unibas.ch/dok/A6390884

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How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection.; We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq.; Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes.; Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig J)
UniBasel Contributors:Seelig, Joachim
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Nov 2017 12:16
Deposited On:03 Jul 2015 08:53

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