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The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis

Cajanek, Lukas and Glatter, Timo and Nigg, Erich A.. (2015) The E3 ubiquitin ligase Mib1 regulates Plk4 and centriole biogenesis. Journal of Cell Science, 128 (9). pp. 1674-1682.

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Official URL: http://edoc.unibas.ch/dok/A6373469

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Abstract

Centrioles function as core components of centrosomes and as basal bodies for the formation of cilia and flagella. Thus, effective control of centriole numbers is essential for embryogenesis, tissue homeostasis, and genome stability. In mammalian cells, the centriole duplication cycle is governed by Polo-like kinase 4 (Plk4). Here we identify the E3 ubiquitin ligase Mind bomb (Mib1) as a novel interaction partner of Plk4. We show that Mib1 localizes to centriolar satellites but redistributes to centrioles in response to conditions that induce centriole amplification. The E3 ligase activity of Mib1 triggers ubiquitination of Plk4 on multiple sites, causing the formation of Lys11-, Lys29- and Lys48-ubiquitin linkages. These modifications control the abundance of Plk4 and its ability to interact with centrosomal proteins, thus counteracting centriole amplification induced by excess Plk4. Collectively, these results identify the interaction between Mib1 and Plk4 as a novel important element in the control of centriole homeostasis.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Cell Biology (Nigg)
UniBasel Contributors:Nigg, Erich A.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Company of Biologists
ISSN:0021-9533
e-ISSN:1477-9137
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:09 Nov 2017 15:56
Deposited On:05 Jun 2015 08:52

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