De novo protein structure determination from near-atomic-resolution cryo-EM maps

Wang, Ray Yu-Ruei and Kudryashev, Mikhail and Li, Xueming and Egelman, Edward H. and Basler, Marek and Cheng, Yifan and Baker, David and DiMaio, Frank. (2015) De novo protein structure determination from near-atomic-resolution cryo-EM maps. Nature methods, Vol. 12, H. 4 , S. 335–338.

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Official URL: http://edoc.unibas.ch/dok/A6357831

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We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Infection Biology (Basler)
UniBasel Contributors:Basler, Marek and Kudryashev, Mikhail
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Jun 2015 08:52
Deposited On:05 Jun 2015 08:52

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