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Yersinia enterocolitica Type III secretion injectisomes form regularly spaced clusters which incorporate new machines upon activation

Kudryashev, Mikhail and Diepold, Andreas and Amstutz, Marlise and Armitage, Judith P. and Stahlberg, Henning and Cornelis, Guy R.. (2015) Yersinia enterocolitica Type III secretion injectisomes form regularly spaced clusters which incorporate new machines upon activation. Molecular microbiology, Vol. 95, H. 5. pp. 875-884.

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Official URL: http://edoc.unibas.ch/dok/A6338894

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Abstract

Bacterial type III secretion systems or injectisomes are multi protein complexes directly transporting bacterial effector proteins into eukaryotic host cells. To investigate the distribution of injectisomes in the bacterium and the influence of activation of the system on that distribution, we combined in vivo fluorescent imaging and high resolution in situ visualization of Yersinia enterocolitica injectisomes by cryo-electron tomography. Fluorescence microscopy showed the injectisomes as regularly distributed spots around the bacterial cell. Under secreting conditions (absence of Ca(2+) ), the intensity of single spots significantly increased compared to non-secreting conditions (presence of Ca(2+) ), in line with an overall up-regulation of expression levels of all components. Single injectisomes observed by cryo electron tomography tended to cluster at distances less than 100 nm, suggesting that the observed fluorescent spots correspond to evenly distributed clusters of injectisomes, rather than single injectisomes. The up-regulation of injectisome components led to an increase in the number of injectisomes per cluster rather than the formation of new clusters. We suggest that injectisome clustering may allow more effective secretion into the host cells.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Blackwell
ISSN:0950-382X
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:05 Jun 2015 08:52
Deposited On:05 Jun 2015 08:52

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