The scaffolding domain of caveolin 2 is responsible for its Golgi localization in Caco-2 cells

Breuza, L. and Corby, S. and Arsanto, J. -P. and Delgrossi, M. -H. and Scheiffele, P. and Le Bivic, A.. (2002) The scaffolding domain of caveolin 2 is responsible for its Golgi localization in Caco-2 cells. Journal of cell science, Vol. 115, H. 23. pp. 4457-4467.

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Official URL: http://edoc.unibas.ch/dok/A5259860

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In this work, we showed that in Caco-2 cells, a polarized cell line derived from human colon cancer that does not express caveolin 1 (Cav-1), there was no detectable expression of caveolin 2 (Cav-2). When Cav-2 was reintroduced in these cells, it accumulated in the Golgi complex. A chimera, in which the scaffolding domain of Cav-1 was replaced by the one from Cav-2, induced a prominent Golgi staining of Cav-1, strongly indicating that this domain was responsible for the accumulation of Cav-2 in the Golgi complex. Cav-2 was able to interact with Cav-1 in the Golgi complex but this interaction was not sufficient to export it from this compartment. Several chimeras between Cav-1 and 2 were used to show that surface expression of caveolin was necessary but not sufficient to promote caveolae formation. Interestingly, levels of incorporation of the chimeras into Triton insoluble rafts correlated with their ability to trigger caveolae formation raising the possibility that a critical concentration of caveolins to discrete domains of the plasma membrane might be necessary for caveolae formation.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Neurobiology > Cell Biology (Scheiffele)
UniBasel Contributors:Scheiffele, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Company of Biologists
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:08 Jun 2012 06:45
Deposited On:22 Mar 2012 13:23

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