Cracks in dentin and enamel after cryopreservation

Kuhl, S. and Deyhle, H. and Zimmerli, M. and Spagnoli, G. and Beckmann, F. and Muller, B. and Filippi, A.. (2012) Cracks in dentin and enamel after cryopreservation. Oral surgery, oral medicine, oral pathology, oral radiology, Vol. 113, H. 2 , e5-e10.

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Official URL: http://edoc.unibas.ch/dok/A6338104

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OBJECTIVE: The objective of this study was to investigate if cryopreservation of teeth for long-term storage leads to cracks in enamel and dentin. STUDY DESIGN: Three teeth, which were extracted for orthodontic reasons, were cryopreserved in liquid nitrogen (temperature -196 degrees C) and thawed according to standard protocols after 4 months. Micro computed tomography using synchrotron radiation was performed to detect cracks in the tooth hard tissues. RESULTS: Cracks were found in the enamel of all teeth, which are associated with forceps application during extraction. Cracks with a width larger than 0.8 mum were not identified in dentin and cementum. CONCLUSION: Although cryopreservation of teeth according to the standard protocol does not generate cracks more than 0.8 mum wide, the use of forceps can result in prominent cracks.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Oncology Surgery (Spagnoli)
UniBasel Contributors:Spagnoli, Giulio C.
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2015 09:14
Deposited On:10 Apr 2015 09:14

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