Assessment of Rho GTPase signaling during neurite outgrowth

Feltrin, D. and Pertz, O.. (2012) Assessment of Rho GTPase signaling during neurite outgrowth. Methods in molecular medicine, Vol. 827. pp. 181-194.

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Official URL: http://edoc.unibas.ch/dok/A6338217

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Rho GTPases are key regulators of the cytoskeleton during the process of neurite outgrowth. Based on overexpression of dominant-positive and negative Rho GTPase constructs, the classic view is that Rac1 and Cdc42 are important for neurite elongation whereas RhoA regulates neurite retraction in response to collapsing agents. However, recent work has suggested a much finer control of spatiotemporal Rho GTPase signaling in this process. Understanding this complexity level necessitates a panel of more sensitive tools than previously used. Here, we discuss a novel assay that enables the biochemical fractionation of the neurite from the soma of differentiating N1E-115 neuronal-like cells. This allows for spatiotemporal characterization of a large number of protein components, interactions, and post-translational modifications using classic biochemical and also proteomics approaches. We also provide protocols for siRNA-mediated knockdown of genes and sensitive assays that allow quantitative analysis of the neurite outgrowth process.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Cell Migration and Neuritogenesis (Pertz)
UniBasel Contributors:Pertz, Olivier
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Humana Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2015 09:14
Deposited On:10 Apr 2015 09:14

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