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High-frequency and adaptive-like dynamics of human CD1 self-reactive T cells

de Lalla, C. and Lepore, M. and Piccolo, F. M. and Rinaldi, A. and Scelfo, A. and Garavaglia, C. and Mori, L. and De Libero, G. and Dellabona, P. and Casorati, G.. (2011) High-frequency and adaptive-like dynamics of human CD1 self-reactive T cells. European journal of immunology, Vol. 41, H. 3. pp. 602-610.

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Official URL: http://edoc.unibas.ch/dok/A6338652

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Abstract

CD1 molecules present lipid antigens to T cells. An intriguing subset of human T cells recognize CD1-expressing cells without deliberately added lipids. Frequency, subset distribution, clonal composition, naive-to-memory dynamic transition of these CD1 self-reactive T cells remain largely unknown. By screening libraries of T-cell clones, generated from CD4(+) or CD4(-) CD8(-) double negative (DN) T cells sorted from the same donors, and by limiting dilution analysis, we find that the frequency of CD1 self-reactive T cells is unexpectedly high in both T-cell subsets, in the range of 1/10-1/300 circulating T cells. These T cells predominantly recognize CD1a and CD1c and express diverse TCRs. Frequency comparisons of T-cell clones from sorted naive and memory compartments of umbilical cord and adult blood show that CD1 self-reactive T cells are naive at birth and undergo an age-dependent increase in the memory compartment, suggesting a naive/memory adaptive-like population dynamics. CD1 self-reactive clones exhibit mostly Th1 and Th0 functional activities, depending on the subset and on the CD1 isotype restriction. These findings unveil the unanticipated relevance of self-lipid T-cell response in humans and clarify the basic parameters of the lipid-specific T-cell physiology.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Experimental Immunology (De Libero)
UniBasel Contributors:De Libero, Gennaro
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Verl. Chemie
ISSN:0014-2980
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2015 09:13
Deposited On:10 Apr 2015 09:13

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