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Oriented lamellar silk fibrous scaffolds to drive cartilage matrix orientation : towards annulus fibrosus tissue engineering

Bhattacharjee, M. and Miot, S. and Gorecka, A. and Singha, K. and Loparic, M. and Dickinson, S. and Das, A. and Bhavesh, N. S. and Ray, A. R. and Martin, I. and Ghosh, S.. (2012) Oriented lamellar silk fibrous scaffolds to drive cartilage matrix orientation : towards annulus fibrosus tissue engineering. Acta Biomaterialia, Vol. 8, H. 9. pp. 3313-3325.

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Official URL: http://edoc.unibas.ch/dok/A6338275

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Abstract

A novel design of silk-based scaffold is developed using a custom-made winding machine, with fiber alignment resembling the anatomical criss-cross lamellar fibrous orientation features of the annulus fibrosus of the intervertebral disc. Crosslinking of silk fibroin fibers with chondroitin sulphate (CS) was introduced to impart superior biological functionality. The scaffolds, with or without CS, instructed alignment of expanded human chondrocytes and of the deposited extracellular matrix while supporting their chondrogenic redifferentiation. The presence of CS crosslinking could not induce statistically significant changes in the measured collagen or glycosaminoglycan content, but resulted in an increased construct stiffness. By offering the combined effect of cell/matrix alignment and chondrogenic support, the silk fibroin scaffolds developed with precise fiber orientation in lamellar form represent a suitable substrate for tissue engineering of the annulus fibrosus part of the intervertebral disc.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:1742-7061
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Apr 2015 09:12
Deposited On:10 Apr 2015 09:12

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