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Knockdown of the centrosomal component SAS-5 results in defects in nuclear morphology in Caenorhabditis elegans

Schmutz, C. and Spang, A.. (2005) Knockdown of the centrosomal component SAS-5 results in defects in nuclear morphology in Caenorhabditis elegans. European Journal of Cell Biology, 84 (1). pp. 75-82.

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Official URL: http://edoc.unibas.ch/dok/A5259751

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Abstract

Several different processes must be completed in order to proceed through cell division. First, the centrosomes have to be duplicated and the genomic material is replicated. The separation of the chromatin is achieved by a bipolar spindle, which in turn is organized by the two centrosomes. The last step of cell division involves the separation of cellular content and the cleavage of the cell by cytokinesis. We used RNAi to study the centrosomal component SAS-5 in the early Caenorhabditis elegans embryo. While the first cell division and the establishment of polarity of sas-5 dsRNA-treated embryos was indistinguishable from wild type, subsequent cleavages were abnormal. Time-lapse microscopy studies of worms expressing beta-tubulin::GFP revealed that the absence of SAS-5 results in a failure of mitotic spindle assembly starting at the two-cell stage embryo. Furthermore, the chromatin in at least one of the two cells in the early embryo was dispersed. Yet, this dispersion did neither trigger apoptosis nor affect nuclear envelope assembly. No intrinsic size control for the nucleus seems to exist in the early embryo.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Growth & Development > Biochemistry (Spang)
UniBasel Contributors:Spang, Anne
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0171-9335
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Nov 2017 15:39
Deposited On:22 Mar 2012 13:23

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