Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange

Brehmer, D. and Rudiger, S. and Gassler, C. S. and Klostermeier, D. and Packschies, L. and Reinstein, J. and Mayer, M. P. and Bukau, B.. (2001) Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange. Nature Structural biology, Vol. 8, H. 5. pp. 427-432.

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Official URL: http://edoc.unibas.ch/dok/A5259716

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The Hsp70 chaperone activity in protein folding is regulated by ATP-controlled cycles of substrate binding and release. Nucleotide exchange plays a key role in these cycles by triggering substrate release. Structural searches of Hsp70 homologs revealed three structural elements within the ATPase domain: two salt bridges and an exposed loop. Mutational analysis showed that these elements control the dissociation of nucleotides, the interaction with exchange factors and chaperone activity. Sequence variations in the three elements classify the Hsp70 family members into three subfamilies, DnaK proteins, HscA proteins and Hsc70 proteins. These subfamilies show strong differences in nucleotide dissociation and interaction with the exchange factors GrpE and Bag-1.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Klostermeier)
UniBasel Contributors:Klostermeier, Dagmar
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Co
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:21
Deposited On:22 Mar 2012 13:23

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